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位置: 首页 > 甲基化/乙酰化抗体 > Histone H3R8me2a Polyclonal Antibody

Histone H3R8me2a Polyclonal Antibody#HW025

Histone H3R8me2a Polyclonal Antibody
Histone H3R8me2a Polyclonal Antibody
Histone H3R8me2a Polyclonal Antibody
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产品总价:
产品详情

产品名称Histone H3R8me2a Polyclonal Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits and were purified by antigen affinity-chromatography.

应用WB,IHC,IF

种属反应性Human,Mouse

免疫原类型Peptide

免疫原描述A synthetic methylated peptide corresponding to residues surrounding R8 of human histone H3

基因/蛋白名称Histone H3

修饰Methyl

别名HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;

数据库入口号Gene ID: 8290 Swiss Prot: Q16695

实际分子量15kDa

浓度1.0mg/ml

配方Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

保存Store at -20�?or -80�? Avoid freeze / thaw cycles.

应用详情

WB 1:500 - 1:2000

IHC 1:50 - 1:200

IF 1:50 - 1:200

Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R8 antibody.
Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R8 antibody. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R8 antibody.
背景

 Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

 

1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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