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位置: 首页 > 抗体 > Zap-70(Ab-493) Antibody

Zap-70(Ab-493) Antibody#21174

Zap-70(Ab-493) Antibody
Zap-70(Ab-493) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Zap-70(Ab-493) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of total Zap-70 protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa.491~495 (S-Y-Y-T-A) derived from Human ZAP70.

基因/蛋白名称Zap-70

别名70 kDa zeta-associated protein; SRK; Syk-related tyrosine kinase; ZA70; ZAP-70

数据库入口号Swiss-Prot: P43403
NCBI Protein: NP _001070.2

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 70kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from HL60 cells using Zap-70(Ab-493) Antibody #21174.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Zap-70(Ab-493) Antibody #21174(left) or the same antibody preincubated with blocking peptide(right).
背景
ZAP70 is a 70-kD tyrosine phosphoprotein that associates with the zeta chain and undergoes tyrosine phosphorylation following TCR stimulation. The ZAP70 gene is expressed in T- and natural KILLER cells. Protein-Tyrosine Kinases (PTKs) play an integral role in T-cell activation. Stimulation of the T-cell antigen receptor results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR-zeta chain, which can mediate the transduction of extracellular stimuli into cellular effector functions

Chan AC, et al. (1995) EMBO J; 14(11): 2499-2508
Kong G, et al. (1996) Mol Cell Biol; 16(9): 5026-5035
Salomon AR, et al. (2003)Proc Natl Acad Sci U S A; 100(2): 443-448

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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