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位置: 首页 > 磷酸化抗体 > SREBP-1 (Phospho-Ser439) Antibody

SREBP-1 (Phospho-Ser439) Antibody#11951

SREBP-1 (Phospho-Ser439) Antibody
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产品总价:
产品详情

产品名称SREBP-1 (Phospho-Ser439) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB,IHC IF

种属反应性Hu

特异性The antibody detects endogenous level of SREBP-1 only when phosphorylated at serine 439.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine439(Q-S-S(p)-P-L) derived from Human SREBP-1 .

基因/蛋白名称SREBP-1

修饰Phospho

别名SRBP1; SREBF1; SREBP1; sterol regulatory element-binding protein 1;

数据库入口号Swiss-Prot#: P36956;
NCBI Gene#: 6720;
NCBI Protein#: NP_001005291.1

UniprotP36956

实际分子量122kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

IHC 1:100 - 1:300

IF 1:50-200

Western blot analysis of extracts from Jurkat cells treated with TNF using Phospho-SREBP-1 (Ser439) antibody #11951.The lane on the right is treated with the antigen-specific peptide.
背景

Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway By similarity. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').

Bengoechea-Alonso MT, Ericsson J (2006) Cell Cycle 5, 1708-18

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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