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位置: 首页 > 磷酸化抗体 > YB1 (Phospho-Ser102) Antibody

YB1 (Phospho-Ser102) Antibody#11819

YB1 (Phospho-Ser102) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称YB1 (Phospho-Ser102) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of YB1 only when phosphorylated at serine 102.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 102(L-R-S(p)-V-G) derived from Human YB1.

基因/蛋白名称YB1

修饰Phospho

别名CBF-A; NSEP1; EFI-A; MSY-1; YBX1

数据库入口号Swiss-Prot#: P67809;
NCBI Gene#: 4904;
NCBI Protein#: NP_004550.2.

UniprotP67809

实际分子量36kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from HepG2 cells treated with PMA using YB1 (Phospho-Ser102) Antibody #11819.The lane on the right is treated with the antigen-specific peptide.
背景

YB-1 is a nuclear protein that binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors CCAAT-containing Y-box of HLA class II genes. Component of cytoplasmic messenger ribonucleoprotein particles (mRNPs). Interacts with AKT1, SFRS9, THOC4, MSH2, XRCC5, WRN and NCL. Can bind to DNA as a homomeric form, (EFI-A)n or as a heteromeric form in association with EFI-B. Homodimer in the presence of ATP.

Sakura H., Gene 73:499-507(1988).
Didier D.K. Proc. Natl. Acad. Sci. U.S.A. 85:7322-7326(1988).
Kolluri R., Nucleic Acids Res. 19:4771-4771(1991).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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