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位置: 首页 > 磷酸化抗体 > SYK (Phospho-Tyr352) Antibody

SYK (Phospho-Tyr352) Antibody#11827

SYK (Phospho-Tyr352) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称SYK (Phospho-Tyr352) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB,ELISA

种属反应性Human,Mouse,Rat

特异性The antibody detects endogenous levels of SYK only when phosphorylated at tyrosine 352.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 352(S-P-Y(p)-A-D) derived from Human SYK.

基因/蛋白名称SYK

修饰Phospho

别名SYK; KSYK; FLJ25043; FLJ37489;

数据库入口号Swiss-Prot#: P43405;
NCBI Gene#: 6850;
NCBI Protein#: NP_001167638.1.

UniprotP43405

实际分子量72kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from HuvEc cells (Lane 2) and HepG2 cells (Lane 3), using SYK (Phospho-Tyr352) Antibody #11827. The lane on the left is treated with antigen-specific peptide.
背景

Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion

Yagi S., Biochem. Biophys. Res. Commun. 200:28-34(1994).
Law C.-L., J. Biol. Chem. 269:12310-12319(1994).
 

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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