免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > 磷酸化抗体 > Elk-1(Phospho-Ser383) Antibody

Elk-1(Phospho-Ser383) Antibody#11004

Elk-1(Phospho-Ser383) Antibody
Elk-1(Phospho-Ser383) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Elk-1(Phospho-Ser383) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of Elk-1 only when phosphorylated at serine 383.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 383 (T-L-S(p)-P-I) derived from Human Elk-1.

基因/蛋白名称Elk-1

修饰Phospho

别名ELK1; ETS-domain protein Elk-1;

数据库入口号Swiss-Prot: P19419
NCBI Protein: NP_001107595.1

UniprotP19419

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 47kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from HT29 cells untreated or treated with UV using Elk-1(Phospho-Ser383) Antibody #11004.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk-1(Phospho-Ser383) Antibody #11004(left) or the same antibody preincubated with blocking peptide(right).
背景
Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. lternatively spliced transcript variants encoding the same protein have been found for this gene.

Xing J, et al. (1996) Science. 273(5277): 959-963.
Janknecht R, et al. (1993) EMBO J. 12(13): 5097-5104.
Marais R, et al. (1993) Cell 73: 381-393.
Kortenjann M, et al. (1994) Mol Cell Biol. 14: 4815-4824.
Cavigelli M, et al. (1995) EMBO J. 14: 5957-5964.

如果您使用该产品11004发表了文章,请通知我们,让我们可以引用您的文献。

5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com