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Smad2(Ab-220) Antibody#21323

Smad2(Ab-220) Antibody
Smad2(Ab-220) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Smad2(Ab-220) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of total Smad2 protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa.218~222 (P-E-T-P-P) derived from Human Smad2.

基因/蛋白名称Smad2

别名JV18-1; MADH2; MADR2; Mad-related protein 2; Mothers against DPP homolog 2

数据库入口号Swiss-Prot: Q15796
NCBI Protein: NP _001003652.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293 and 3T3 cells using Smad2(Ab-220) Antibody #21323
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Smad2(Ab-220) Antibody #21323(left) or the same antibody preincubated with blocking peptide(right).
背景
Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.

Sang Gyun Kim et al. (2004) Mol Biol Cell. February; 15(2): 420

如果您使用该产品21323发表了文章,请通知我们,让我们可以引用您的文献。

et al,TGF β1 mediated Smad signaling pathway and EMT in hepatic fibrosis induced by Nano NiO in vivo and in vitro.In Environ Toxicol on 2020 Apr by Zhang Q, Chang X ,et al..PMID: 31737983, , (2020),
PMID: 31737983
et al,Distribution of Smad mRNA and proteins in the rat brain.In J Chem Neuroanat. On 2018 Jul by Nakajima T, Hata R et al..PMID: 29196107, , (2018),
PMID: 29196107
et al,Temporal and regional patterns of Smad activation in the rat hippocampus following global ischemia.In J Neurol Sci on 2014 Feb 15 by Takayuki Nakajima , Masafumi Yanagihara et al ..PMID:24290497, , (2014),
PMID: 24290497
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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