产品详情
产品名称Parkin Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB IHC
种属反应性Hu Ms Rt
特异性The antibody detects endogenous levels of total Parkin protein.
免疫原类型Peptide
免疫原描述Synthesized peptide derived from human Parkin antibody.
基因/蛋白名称Parkin
别名PARK2; PRKN; PRKN2; Parkinson disease protein 2; Parkinson juvenile disease protein 2
数据库入口号Swiss-Prot: O60260
NCBI Gene ID: 5071
UniprotO60260
实际分子量52kd
浓度1.0mg/ml
配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunohistochemical analysis of paraffin-embedded human brain tissue using Parkin antibody #33464.
Western blot analysis of extracts from Jukat cells, using Parkin antibody #33464.
Western blot analysis of extracts from Jurkat cells using Parkin antibody #33464. The lane on the left is treated with synthesized peptide.
背景
Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins, such as BCL2, SYT11, CCNE1, GPR37, STUB1, a 22 kDa O-linked glycosylated isoform ofSNCAIP, SEPT5, ZNF746 and AIMP2. Mediates monoubiquitination as well as 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context. Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating 'Lys-63'-linked polyubiquitination of misfolded proteins such as PARK7: 'Lys-63'-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation. Mediates 'Lys-63'-linked polyubiquitination of SNCAIP, possibly playing a role in Lewy-body formation. Mediates monoubiquitination of BCL2, thereby acting as a positive regulator of autophagy. Promotes the autophagic degradation of dysfunctional depolarized mitochondria (mitophagy), potentially by the ubiquitination of mitochondrial proteins. Mediates 'Lys-48'-linked polyubiquitination of ZNF746, followed by degradation of ZNF746 by the proteasome; possibly playing a role in the regulation of neuron death. Limits the production of reactive oxygen species (ROS). Regulates cyclin-E during neuronal apoptosis. In collaboration with CHPF isoform 2, may enhance cell viability and protect cells from oxidative stress. Independently of its ubiquitin ligase activity, protects from apoptosis by the transcriptional repression of p53/TP53. May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity. May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. May represent a tumor suppressor gene.
Fang Yang, J. Biol. Chem., Apr 2005; 280: 17154 - 17162.
Yukiko Kuroda, Hum. Mol. Genet., Mar 2006; 15: 883 - 895.
Iris H. Henn, J. Neurosci., Feb 2007; 27: 1868 - 1878.
Monica Joch, Mol. Biol. Cell, Jun 2007; 10.1091/mbc.E05-11-1027.
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