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位置: 首页 > 抗体 > XRCC4 Antibody

XRCC4 Antibody #33549

XRCC4 Antibody
是否有货: 有货
产品总价:
产品详情

产品名称XRCC4 Antibody

来源种属Rabbit

克隆性Polyclonal

纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

应用WB

种属反应性Hu

特异性The antibody detects endogenous levels of total XRCC4 protein.

免疫原类型Peptide

免疫原描述Synthesized peptide derived from Internal of human XRCC4.

基因/蛋白名称XRCC4

别名DNA-repair protein XRCC4; X-ray repair cross-complementing protein 4;

数据库入口号Swiss-Prot: Q13426
NCBI Gene ID: 7518

UniprotQ13426

实际分子量40kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
Western blotting: 1:500~1:3000

Western blot analysis of extracts from COLO205 cells, using XRCC4 antibody #33549.
背景
Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

Ling Chen, J. Biol. Chem., Aug 2000; 275: 26196.
J¨¦r?me Drouet, J. Biol. Chem., Feb 2005; 280: 7060 - 7069.
Ulf Grawunder, J. Biol. Chem., Sep 1998; 273: 24708.
Ray Leber, J. Biol. Chem., Jan 1998; 273: 1794.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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