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Grp78 Antibody#35394

Grp78 Antibody
Grp78 Antibody
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产品总价:
产品详情

产品名称Grp78 Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were purified by antigen-affinity chromatography.

应用WB

种属反应性Hu Ms

特异性The antibody detects endogenous levels of total Grp78 protein.

免疫原类型Recombinant Protein

免疫原描述Recombinant fragment corresponding to a region within amino acids 478 and 654 of Grp78.

基因/蛋白名称Grp78

别名BIP antibody; FLJ26106 antibody; GRP78 antibody; MIF2 antibody; HSPA5 antibody; immunoglobulin heavy chain-binding protein antibody; 78 kDa glucose-regulated protein antibody; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody; "heat shoc

数据库入口号Swiss-Prot#:P11021;
NCBI Gene#:3309

UniprotP11021

实际分子量72kd

浓度1mg/ml

配方Rabbit IgG in 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

保存Store at -20˚C

应用详情
Western blotting: 1:500-1:3000
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
#35394 diluted at 1:2000
Grp78 antibody detects Grp78 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
#102567 dilution: 1:1000
背景
When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]

如果您使用该产品35394发表了文章,请通知我们,让我们可以引用您的文献。

et al,Ginsenosides reduce body weight and ameliorate hepatic steatosis in high fat diet?induced obese mice via endoplasmic reticulum stress and p?STAT3/STAT3 signaling.In Mol Med Rep on 2020 Mar; by Yao Y et al..PMID:32016448, , (2020),
PMID: 32016448
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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