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位置: 首页 > 标签抗体 > FLAG tag Antibody

FLAG tag Antibody#35535

FLAG tag Antibody
是否有货: 有货
产品总价:
产品详情

产品名称FLAG tag Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antigen affinity purification.

应用ELISA WB

特异性The antibody detects transfected proteins contanining FLAG tag.

免疫原类型Peptide

免疫原描述Synthetic peptide: DYKDDDDK conjugated to KLH

基因/蛋白名称FLAG tag

别名DDDDK epitope tag; DYKDDDDK epitope tag

实际分子量50kDa

浓度1.0 mg/ml

配方Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

保存Store at -20˚C

应用详情
ELISA: 1:5000-1:10000
Western blotting: 1:5000-1:20000
Gel: 8%SDS-PAGELysate: 0.08/0.1/0.12/0.16/0.2 ugLane 1-5: 0.08/0.1/0.12/0.16/0.2 ug Fusion proteins containing the Flag TagPrimary antibody: at dilution 1/8000Secondary antibody: at 1/5000 dilutionExposure time: 10 seconds
背景

The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins. (The background information is obtained from official databases and does not represent the product information.)

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et al,Interaction between NS1 and Cellular MAVS Contributes to NS1 Mitochondria Targeting. In Viruses on 2021 Sep 23 by Yeu-Yang Tseng, Chih-Ying Kuan,et al..PMID:34696339, , (2021),
PMID: 34696339
et al,MLL2 promotes cancer cell lymph node metastasis by interacting with RelA and facilitating STC1 transcription.In Cell Signal on 2020 Jan; by Li H, Li Q, et al..PMID:31676369, , (2020),
PMID: 31676369
et al,O-GlcNAcylation of PFKFB3 is required for tumor cell proliferation under hypoxia.In Oncogenesis on 2020 Feb 14 by Lei Y, Chen T, et al..PMID:32060258, , (2020),
PMID: 32060258
et al,Upregulation of ZNF148 in SDHB-deficient gastrointestinal stromal tumor potentiates Forkhead box M1-mediated transcription and promotes tumor cell invasion. In Cancer Sci on 2020 Apr by Gao X, Ma C, et al..PMID: 32060966, , (2020),
PMID: 32060966
et al,PAK4 Phosphorylates Fumarase and Blocks TGFβ-Induced Cell Growth Arrest in Lung Cancer Cells. In Cancer Res on 2019 Apr 1 by Chen T, Wang T, et al..PMID:30683654, , (2019),
PMID: 30683654
TaoChen, Jingjie Li et al, et al,PKC蔚 phosphorylates MIIP and promotes colorectal cancer metastasis through inhibition of RelA deacetylation., Nature Communications, 8(1):939. doi: 10.1038/s41467-017-01024-2. (2017 Oct 16),
PMID: 29038521
Ting Wang锛孮iujing Yu Li et al et al,GlcNAcylation of fumarase maintains tumour growth under glucose deficiency., Nature Cell Biology, doi: 10.1038/ncb3562 (2017),
PMID: 28628081
查看更多
et al,O-GlcNAcylation of Fumarase Maintains Tumour Growth Under Glucose Deficiency .In Nat Cell Biol ON 2017 Jul by Ting Wang , Qiujing Yu,et al..PMID: 28628081, , (2017),
PMID: 28628081
et al,PKC蔚 phosphorylates MIIP and promotes colorectal cancer metastasis through inhibition of RelA deacetylation.In Nat Commun on 2017 Oct 16 by Tao Chen, Jingjie Li ,et al..PMID: 29038521, , (2017),
PMID: 29038521
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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