产品详情
产品名称Akt(Phospho-Thr308) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC IF
种属反应性Hu Ms Rt
特异性The antibody detects endogenous level of Akt only when phosphorylated at threonine 308.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of threonine 308 (M-K-T(p)-F-C) derived from Human Akt.
基因/蛋白名称Akt
修饰Phospho
别名C-AKT; PKB; PKB-alpha; RAC; RAC-PK-alpha
数据库入口号Swiss-Prot: P31749
NCBI Protein: NP_001014431.1
UniprotP31749
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Hela cells untreated or treated with EGF, serum or both using Akt(Phospho-Thr308) Antibody #11055.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (Phospho-Thr308) Antibody #11055 (left) or the same antibody preincubated with blocking peptide #51055 (right).
Immunofluorescence staining of methanol-fixed Hela cells showing nuclear dot staining using Akt(Phospho-Thr308) Antibody #11055.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (Phospho-Thr308) Antibody #11055.
Immunofluorescence staining of methanol-fixed Hela cells showing nuclear dot staining using Akt (Phospho-Thr308) Antibody #11055.
背景
General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
Tremblay F, et al. (2005)Diabetes; 54(9): 2674-84.
Xu BE, et al. (2005)J Biol Chem; 280(40): 34218-23.
Samuels Y, et al. (2005)Cancer Cell; 7(6): 561-73.
Di Maira G, et al. (2005)Cell Death Differ; 12(6): 668-77.
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