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NFκB p65 Mouse Monoclonal Antibody#38054

NFκB p65 Mouse Monoclonal Antibody
NFκB p65 Mouse Monoclonal Antibody
NFκB p65 Mouse Monoclonal Antibody
是否有货: 有货
产品总价:
产品详情

产品名称NFκB p65 Mouse Monoclonal Antibody

来源种属Mouse

克隆性Monoclonal

纯化Affinity purification using immunogen.

应用WB IF IP

种属反应性Hu Rt Ms

特异性NFκB p65 Mouse monoclonal antibody detects endogenous p65 proteins.

基因/蛋白名称NFκB p65

别名MGC131774; NFKB3; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65; RELA; TF65; Transcription factor p65

数据库入口号Swiss-Prot#:Q04206

UniprotQ04206

实际分子量65kd

浓度1.0mg/ml

配方Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
Western blotting: 1:1000~1:3000
Immunofluorescence: 1:100
Immunoprecipitation: 1:200
Western blot analysis of 1) Hela, 2) Rat Heart Tissue, 3) Mouse Spleen Tissue, using #38054 diluted at 1:2000.
IF analysis of Hela with #38054(Left) and DAPI (Right) diluted at 1:100.
1¡¢Input: Hela Cell Lysate
2¡¢IP product: IP dilute 1:200
Western blot analysis: primary antibody :#38054 1:2,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000
背景
NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.

如果您使用该产品38054发表了文章,请通知我们,让我们可以引用您的文献。

et al,Ischemic postconditioning protects against acute kidney injury after limb ischemia reperfusion by regulating HMGB1 release and autophagyIn Ren FailOn2023 DecbyZhongdi Liu , Yifan Chen et al..PMID:37158301, , (2023),
PMID: 37158301
et al,Costunolide improved dextran sulfate sodium-induced acute ulcerative colitis in mice through NF-kB, STAT1/3, and Akt signaling pathways. In Int Immunopharmacol on 2020 Jul by Fan Xie, Hai Zhang, et al..PMID:32413737, , (2020),
PMID: 32413737
et al,Astragalus polysaccharides mediated preventive effects on bronchopulmonary dysplasia in rats.In Pediatr Res on 2014 Oct by Xiao-Hong Wang, Hong-Ling Jia et al..PMID: 25029259 , , (2014),
PMID: 25029259
et al,The antioxidant effects of garlic saponins protect PC12 cells from hypoxia-induced damage. In Br J Nutr on 2011 Apr by Hong Luo, Jian Huang, et al..PMID: 21205417, , (2011),
PMID: 21205417
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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