免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > 磷酸化抗体 > p53(Phospho-Ser315) Antibody

p53(Phospho-Ser315) Antibody#11100

p53(Phospho-Ser315) Antibody
p53(Phospho-Ser315) Antibody
p53(Phospho-Ser315) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称p53(Phospho-Ser315) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu

特异性The antibody detects endogenous level of p53 only when phosphorylated at serine 315.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 315 (S-S-S(p)-P-Q) derived from Human p53.

基因/蛋白名称p53

修饰Phospho

别名Tumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13; TP53;

数据库入口号Swiss-Prot: P04637
NCBI Protein: NP_000537.3

UniprotP04637

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 53kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Hela cells using p53(Phospho-Ser315) Antibody #11100(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53(Phospho-Ser315) Antibody #11100(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using p53(Phospho-Ser315) Antibody #11100.
背景
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.

Lu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934.
Lohrum, M. et.al. (1996) Oncogene 13, 2527-2539.
Posp

如果您使用该产品11100发表了文章,请通知我们,让我们可以引用您的文献。

et al,Cell cycle arrest and apoptosis of OVCAR-3 and MCF-7 cells induced by co-immobilized TNF-伪 plus IFN-纬 on polystyrene and the role of p53 activation. In Biomaterials on 2012 Sep by Yan-Qing Guan, Zhibin Li, et al..PMID: 22682938, , (2012),
PMID: 22682938
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com