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位置: 首页 > 磷酸化抗体 > Tau(Phospho-Ser404) Antibody

Tau(Phospho-Ser404) Antibody#11112

Tau(Phospho-Ser404) Antibody
Tau(Phospho-Ser404) Antibody
Tau(Phospho-Ser404) Antibody
Tau(Phospho-Ser404) Antibody
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产品总价:
产品详情

产品名称Tau(Phospho-Ser404) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of Tau only when phosphorylated at serine 404.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 404 (D-T-S(p)-P-R) derived from Human Tau.

基因/蛋白名称Tau

修饰Phospho

别名MAPT; MTAPT; MTBT1; Neurofibrillary tangle protein; PHF-tau

数据库入口号Swiss-Prot: P10636
NCBI Protein: NP_001116538.1

UniprotP10636

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 48 62 78 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Immunohistochemical analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer
Immunofluorescence staining of methanol-fixed Hela cells using Tau(Phospho-Ser404) Antibody #11112.
Western blot analysis of extracts from C6 cells, treated with calf intestinal phosphatase (CIP), using Tau (Phospho-Ser404) Antibody #11112.
Western blot analysis of extracts from C6 cells using Tau(Phospho-Ser404) Antibody #11112.
背景

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Li G, Yin H, et.al .(2004) J Biol Chem ; 279(16): 15938-45.
Noble W, et al. (2003) Neuron ; 38(4): 555-65.
Giasson BI, et al.(2002). Biochemistry; 41(51): 15376-87.
Lee G., et.al. (1989). Neuron 2:1615-1624.
Andreadis A.et.al. (1992) Biochemistry 31:10626-10633.

如果您使用该产品11112发表了文章,请通知我们,让我们可以引用您的文献。

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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