产品详情
产品名称FKBP12 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性JM11-09
纯化ProA affinity purified
应用WB, ICC/IF
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名12 kDa FK506-binding protein antibody
12 kDa FKBP antibody
Calstabin 1 antibody
FK506 binding protein 1 antibody
FK506 binding protein 12 antibody
FK506 binding protein 1A (12kD) antibody
FK506 binding protein 1A 12kDa antibody
FK506 binding protein 1A antibody
FK506 binding protein T cell 12 kD antibody
FK506 binding protein, T cell, 12 kD antibody
FK506 binding protein12 antibody
FK506-binding protein 1A antibody
FKB1A_HUMAN antibody
FKBP 12 antibody
FKBP 1A antibody
FKBP-12 antibody
FKBP-1A antibody
FKBP1 antibody
FKBP12 antibody
FKBP12 Exip3 antibody
FKBP12C antibody
fkbp1a antibody
Immunophilin FKBP12 antibody
Peptidyl prolyl cis trans isomerase antibody
Peptidyl-prolyl cis-trans isomerase FKBP1A antibody
PKC12 antibody
PKCI2 antibody
PPIase antibody
PPIase FKBP1A antibody
Protein kinase C inhibitor 2 antibody
Rotamase antibody
数据库入口号Swiss-Prot#:P62942
计算分子量12 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1000
ICC: 1:100-1:500
Western blot analysis of FKBP12 on different cells lysates using anti-FKBP12 antibody at 1/500 dilution. Positive control:
Line 1: SH-SY5Y
Line 2:V937
ICC staining FKBP12 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining FKBP12 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining FKBP12 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
Immunophilins are a highly conserved family of cis-trans peptidyl-prolyl isomerases which bind to and mediate the effects of immunosuppressive drugs such as Cyclosporin, FK506 and Rapamycin. FKBP12.6, also known as FK506-binding protein 1B, is a 108 amino acid immunophilin belonging to the FKBP-type PPIase family. Subcellularly localized to the cytoplasm, FKBP12.6 binds to RyR in cardiac muscle sarcoplasmic reticulum and possibly plays a unique physiological role in excitation-contraction coupling in cardiac muscle. FKBP12.6 also catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Ubiquitously expressed, FKBP12.6 is found at highest levels in brain and thymus. FKBP12.6 is expressed as two isoforms produced by alternative splicing.
背景文献
1. Ballister ER et al. Recruitment of Mad1 to metaphase kinetochores is sufficient to reactivate the mitotic checkpoint. J Cell Biol 204:901-8 (2014). 2. Buheitel J & Stemmann O Prophase pathway-dependent removal of cohesin from human chromosomes requires opening of the Smc3-Scc1 gate. EMBO J 32:666-76 (2013).
Yes
