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位置: 首页 > 磷酸化抗体 > Smad1(Phospho-Ser465) Antibody

Smad1(Phospho-Ser465) Antibody#11321

Smad1(Phospho-Ser465) Antibody
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产品总价:
产品详情

产品名称Smad1(Phospho-Ser465) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of Smad1 only when phosphorylated at serine 465.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 465 (I-S-S-V-S(p)) derived from Human Smad1.

基因/蛋白名称Smad1

修饰Phospho

别名 Mad-related protein 1; SMAD 1; hSMAD1; Transforming growth factor-beta-signaling protein 1;

数据库入口号Swiss-Prot: Q15797
NCBI Protein: NP_001003688.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 55kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Hela cells untreated or treated with PMA using Smad1(Phospho-Ser465) Antibody #11321.
背景
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD).

Hiroaki Seto, et al. (2004) J Clin Invest. March 1; 113(5): 718

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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025-58868422

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