免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > 抗体 > Cyclin F Antibody

Cyclin F Antibody#33352

Cyclin F Antibody
Cyclin F Antibody
Cyclin F Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Cyclin F Antibody

来源种属Rabbit

克隆性Polyclonal

纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous levels of total Cyclin F protein.

免疫原类型Peptide

免疫原描述Synthesized peptide derived from human Cyclin F.

基因/蛋白名称Cyclin F

别名FBX1; FBXO1; F-box only protein 1;

数据库入口号Swiss-Prot: P41002
NCBI Gene ID: 899

UniprotP41002

实际分子量88kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin F antibody #33352.
Western blot analysis of extracts from HeLa cells, using Cyclin F antibody #33352.
Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using Cyclin F antiobdy #33352. The lane on the left is treated with systhesized peptide.
背景
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication.

Tsz Kan Fung, J. Biol. Chem., Sep 2002; 277: 35140 - 35149.
Michael T. Tetzlaff, Mol. Cell. Biol., Mar 2004; 24: 2487 - 2498.
Cain H. Yam, Mol. Cell. Biol., Jan 1999; 19: 635 - 645.
Michael T. Tetzlaff, PNAS, Mar 2004; 101: 3338 - 3345.

如果您使用该产品33352发表了文章,请通知我们,让我们可以引用您的文献。

et al,Longikaurin A, a natural ent-kaurane, induces G2/M phase arrest via downregulation of Skp2 and apoptosis induction through ROS/JNK/c-Jun pathway in hepatocellular carcinoma cells.In Cell Death Dis on 2014 Mar 20 by Y-J Liao, H-Y Bai et al..PMID:24651440 , , (2014),
PMID: 24651440
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com