产品详情
产品名称MEK2(Phospho-Thr394) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC IF
种属反应性Human
特异性The antibody detects endogenous level of MEK2 only when phosphorylated at threonine 394.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of threonine 394 (P-G-T(p)-P-T) derived from Human MEK-2.
基因/蛋白名称MEK2
标记Unconjugated
别名ERK activator kinase 2; MAP kinase kinase 2; MAP2K2; MAPK/ERK kinase 2; MAPKK 2
数据库入口号Swiss-Prot: P36507
NCBI Protein: NP_109587.1
Uniprot
P36507
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 44kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from HepG2 and Hela cells untreated or treated with UV using MEK-2(Phospho-Thr394) Antibody #11008.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK-2(Phospho-Thr394) Antibody #11008(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using MEK-2(Phospho-Thr394) Antibody #11008.
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
Crews C M, et al. (1992) Science. 258:478-480.
Alessi D R, et al. (1994) EMBO J. 13:1610-1619.
Rosen L B, et al. (1994) Neuron. 12:1207-1221.
Cowley S, et al. (1994) Cell. 77:841-852.
如果您使用该产品11008发表了文章，请通知我们，让我们可以引用您的文献。
有货
