产品详情
产品名称c-Jun(Phospho-Thr239) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of c-Jun only when phosphorylated at threonine239.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of threonine 239 (G-E-T(p)-P-P) derived from Human c-Jun.
基因/蛋白名称c-Jun
标记Unconjugated
别名AH119; AP1; Jun A; c-Jun; p39
数据库入口号Swiss-Prot: P05412
NCBI Protein: NP_002219.1
Uniprot
P05412
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Hela cells untreated or treated with UV using c-Jun(Phospho-Thr239) Antibody #11024.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun(Phospho-Thr239) Antibody #11024(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using c-Jun(Phospho-Thr239) Antibody #11024.
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.
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Derijard B, et al. (1994) Cell. 76:1025-1037.
Kyriakis J M, et al. (1994) Nature. 369: 156-160.
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et al,Oncogenic Functions of Secreted Frizzled-Related Protein 2 in Human Renal Cancer.
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et al,Oncogenic functions of secreted Frizzled-related protein 2 in human renal cancer. In Mol Cancer Ther on 2010 Jun by Soichiro Yamamura, Kazumori Kawakami, et al..PMID: 20501806
, (2010),
PMID:
20501806
et al,MFHAS1 Suppresses TLR4 Signaling Pathway via Induction of PP2A C Subunit Cytoplasm Translocation and Inhibition of c-Jun Dephosphorylation at Thr239.In Mol Immunol on 2017 Aug by Qiqing Shi , Bo Xiong,et al..PMID: 28609714
, (2017),
PMID:
28609714
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