产品详情
产品名称c-Jun(Phospho-Ser243) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of c-Jun only when phosphorylated at serine 243.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of serine 243 (P-L-S(p)-P-I) derived from Human c-Jun.
基因/蛋白名称c-Jun
标记Unconjugated
别名AH119; AP1; Jun A; c-Jun; p39
数据库入口号Swiss-Prot: P05412
NCBI Protein: NP_002219.1
Uniprot
P05412
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from sorbitol-treated 293, Serum-treated C2C12 and UV-treated HepG2 cells using c-Jun(Phospho-Ser243) Antibody #11025.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun(Phospho-Ser243) Antibody #11025(left) or the same antibody preincubated with blocking peptide(right).
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.
Boyle W J, et al. (1991) Cell. 64(3): 573-584.
Binetruy B, et al. (1991) Nature. 351: 122-127.
Smeal T, et al. (1991) Nature. 354:494-496.
Derijard B, et al. (1994) Cell. 76:1025-1037.
Kyriakis J M, et al. (1994) Nature. 369: 156-160.
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et al,Oncogenic functions of secreted Frizzled-related protein 2 in human renal cancer. In Mol Cancer Ther on 2010 Jun by Soichiro Yamamura, Kazumori Kawakami, et al..PMID: 20501806
, (2010),
PMID:
20501806
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