产品详情
产品名称JunB(Phospho-Ser259) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of JunB only when phosphorylated at serine 259.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of serine 259 (P-V-S(p)-P-I) derived from Human JunB.
基因/蛋白名称JunB
标记Unconjugated
别名AP-1
数据库入口号Swiss-Prot: P17275
NCBI Protein: NP_002220.1
Uniprot
P17275
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from HT29 cells untreated or treated with EGF using JunB(Phospho-Ser259) Antibody #11027.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunB(Phospho-Ser259) Antibody #11027(left) or the same antibody preincubated with blocking peptide(right).
Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.
Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101(33): 12130-12135.
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et al,AP-1 regulates cyclin D1 and c-MYC transcription in an AKT-dependent manner in response to mTOR inhibition: role of AIP4/Itch-mediated JUNB degradation. In Mol Cancer Res on 2011 Jan by Raffi Vartanian, Janine Masri, et al..PMID:21135252
, (2011),
PMID:
21135252
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