产品详情
产品名称STAT1(Phospho-Tyr701) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC IP
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of STAT1 only when phosphorylated at tyrosine 701.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of tyrosine 701 (T-G-Y(p)-I-K) derived from Human STAT1.
基因/蛋白名称STAT1
标记Unconjugated
别名CANDF7, ISGF-3, STAT91
数据库入口号Swiss-Prot: P42224
NCBI Protein: NP _009330.1
Uniprot
P42224
实际分子量84,91kd
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 84,91kd
WB: 1:500~1:1000
IHC: 1:50~1:100IP: 1:50-200
Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120.
Durbin J E, et al. (1996) Cell. 84: 443-450.
Meraz M A, et al. (1996) Cell. 84: 431-442.
Wakao H, et al. (1994) EMBO J. 13: 2182-2191.
Demoulin J, B. et al. (1999) J Biol Chem. 274: 25855-258
Ihle J N, et al. (1994) Trends Biochem Sci. 19: 222-227.
如果您使用该产品11044发表了文章，请通知我们，让我们可以引用您的文献。
et al,High-glucose environment reduces human b-defensin-2 expression in human keratinocytes: implications for poor diabetic wound healing.
, (2012),
PMID:
22283836
et al,Inhibitory effects of chalcone glycosides isolated from?Brassica rapa?L. 隆庐hidabeni隆炉 and their synthetic derivatives on LPS-induced NO production in microglia.
, (2011),
PMID:
21856162
et al,Neural Stem Cells Secrete Factors That Promote Auditory Cell Proliferation Via a Leukemia Inhibitory Factor Signaling Pathway
, (2010),
PMID:
20882565
et al,Chikungunya virus nonstructural protein 2 inhibits type I/II interferon-stimulated JAK-STAT signaling.
, (2010),
PMID:
20686047
et al,Danzhi Jiangtang Capsule ameliorates kidney injury via inhibition of the JAK-STAT signaling pathway and increased antioxidant capacity in STZ-induced diabetic nephropathy rats.In Biosci Trends.On 2019 Jan 22 by Sun M, Bu W et al..PMID: 30606979
, (2018),
PMID:
30606979
et al,Chronic Inflammation In Response to Injury: Retention of Myeloid Cells in Injured Tissue Is Driven by Myeloid Cell IntrInsic Factors. In J Invest Dermatol on 2019 Jan 28 by Torbica T, Wicks K, et al..PMID:30703358
, (2019),
PMID:
30703358
et al,A thiazole-derived oridonIn analogue exhibits antitumor activity by directly and allosterically InhibitIng STAT3. In J Biol Chem on 2019 Nov 15;by Shen X, Zhao L,et al..PMID:31594861
, (2019),
PMID:
31594861
查看更多
et al,The C-terminal Domain of Chikungunya Virus nsP2 Independently Governs Viral RNA Replication, Cytopathicity, and Inhibition of Interferon Signaling.In J Virol on 2013 Sep by Jelke J Fros, Erika van der Maten,et al..PMID:23864632
, (2013),
PMID: 23864632
et al,High-glucose Environment Reduces Human 尾-defensin-2 Expression in Human Keratinocytes: Implications for Poor Diabetic Wound Healing.In
Br J Dermatol on 2012 Jun by C-C E Lan, C-S Wu,et al..PMID:22283836
, (2012),
PMID: 22283836
et al,Neural stem cells secrete factors that promote auditory cell proliferation via a leukemia inhibitory factor signaling pathway. In J Neurosci Res on 2010 Nov 15 by Hsin-Chien Chen, Hsin-I Ma, et al..PMID: 20882565
, (2010),
PMID: 20882565
et al,Chikungunya virus nonstructural protein 2 inhibits type I/II interferon-stimulated JAK-STAT signaling. In J Virol on 2010 Oct by Jelke J Fros, Wen Jun Liu, et al..PMID:
20686047
, (2010),
PMID: 20686047
et al,Inhibitory effects of chalcone glycosides isolated from Brassica rapa L. 鈥榟idabeni锟?and their synthetic derivatives on LPS-induced NO production in microglia. In Bioorg Med Chem on 2011 Sep 15 by Hirokazu Hara, Yoko Nakamura, et al..PMID:21856162
, (2011),
PMID: 21856162
et al,DJC suppresses Advanced Glycation End Products-Induced JAK-STAT signaling and ROS in mesangial cells.In Evid Based Complement Alternat Med on 2017 May 29 by Min Sun, Yan Li ,et al..PMID: 28630633
, (2017),
PMID: 28630633
et al,Inhibitory Effects of Chalcone Glycosides Isolated From Brassica Rapa L. 'Hidabeni' and Their Synthetic Derivatives on LPS-induced NO Production in Microglia.In Bioorg Med Chem on 2011 Sep 15 by Hirokazu Hara, Yoko Nakamura,et al..PMID: 21856162
, (2017),
PMID: 21856162
et al,Newly Synthesized 鈥楬idabeni锟?Chalcone Derivatives Potently Suppress LPS-Induced NO Production via Inhibition of STAT1, but Not NF-魏B, JNK, and p38, Pathways in Microglia.In Biol Pharm Bull On 2014 by Hirokazu Hara , Ryoko Ikeda et al..PMID: 24882415
, (2014),
PMID: 24882415
et al,Molecular analysis of curcumin-induced polarization of murine RAW264. 7 macrophages.In J Cardiovasc Pharmacol on 2014 Jun by Fangyuan Chen , Ning Guo et al..PMID:24709638
, (2014),
PMID: 24709638
et al,Expression of interferon effector gene SART1 correlates with interferon treatment response against hepatitis B infection. In Mediators Inflamm on 2016 by Yong Li, Chuanlong Zhu et al..PMID: 28077916
, (2016),
PMID: 28077916
et al,ycobacterium tuberculosis EspB protein suppresses interferon-纬-induced autophagy in murine macrophages.In J Microbiol Immunol Infect on 2016 Dec by DanDan Huang, Lang Bao et al..PMID:25641595
, (2016),
PMID: 25641595
et al,Costunolide improved dextran sulfate sodium-induced acute ulcerative colitis in mice through NF-kB, STAT1/3, and Akt signaling pathways. In Int Immunopharmacol on 2020 Jul by Fan Xie, Hai Zhang, et al..PMID:32413737
, (2020),
PMID: 32413737
et al,Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase
, (2017),
PMID: 29371942
et al,Identification of Cytochrome P450 2E1 as a Novel Target in Neuroinflammation and Development of Its Inhibitor Q11 as a Treatment Strategy.
, (2025),
PMID: 40122152
有货
