产品详情
产品名称FAK(Phospho-Tyr861) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB
种属反应性Human
特异性The antibody detects endogenous level of FAK only when phosphorylated at tyrosine 861.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of tyrosine 861 (H-I-Y(p)-Q-P) derived from Human FAK.
基因/蛋白名称FAK
标记Unconjugated
别名FADK 1; FAK1; PTK2
数据库入口号Swiss-Prot: Q05397
NCBI Protein: NP _005598.3
Uniprot
Q05397
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 125kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HT29 cells untreated or treated with EGF using FAK(Phospho-Tyr861) Antibody #11059.
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Shi Q, et al. (2003) Mol Biol Cell; 14(10): 4306-15.
Vadlamudi RK, et al. (2003) FEBS Lett; 543(1-3): 76-80.
Eliceiri BP, et al. (2002) J Cell Biol Apr 01; 157(1): 149-60.
Abu-Ghazaleh R, (2001) et al. Biochem J; 360(Pt 1): 255-64.
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Zhenhuan Zheng, Yujun Wei, et al..PMID: 18987023
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et al,Zoledronate inhibits the proliferation, adhesion and migration of vascular smooth muscle cells. In Eur J Pharmacol 2009 Jan 5 by Liang Wu, Lei Zhu, et al..PMID:
19000670
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PMID: 19000670
et al,Anticancer Effect of Heparin-Taurocholate Conjugate on Orthotopically Induced Exocrine and Endocrine Pancreatic Cancer
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