产品详情
产品名称HER2(Phospho-Tyr877) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of HER2 only when phosphorylated at tyrosine 877.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of tyrosine 877 (T-E-Y(p)-H-A) derived from Human HER2.
基因/蛋白名称HER2
标记Unconjugated
别名C-erbB-2; ErbB2;
数据库入口号Swiss-Prot: P04626
NCBI Protein: NP_001005862.1
Uniprot
P04626
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 185kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from A431 cells untreated or treated with EGF using HER2(Phospho-Tyr877) Antibody #11075.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HER2(Phospho-Tyr877) Antibody #11075(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF7 cells using HER2(Phospho-Tyr877) Antibody #11075.
Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-a and amphiregulin.
Dittadi, R. et al. (2000) J. Natl. Cancer Inst. 92, 1443-1444.
Muthuswamy, S. K. et al. (1999) Mol. Cell. Biol. 19, 6845-6857.
Qian, X. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 1500-1504.
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et al,EGF promotes neuroendocrine-like differentiation of prostate cancer cells in the presence of LY294002 through increased ErbB2 expression independent of the phosphatidylinositol 3-kinase-AKT pathway
, (2012),
PMID:
22461520
et al,EGF promotes neuroendocrine-like differentiation of prostate cancer cells in the presence of LY294002 through increased ErbB2 expression independent of the
phosphatidylinositol 3-kinase-AKT pathway. In Carcinogenesis on 2012 Jun by Javier Angulo, Pilar L贸pez-Ruiz, et al..PMID: 22461520
, (2012),
PMID:
22461520
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