产品详情
产品名称EGFR(Phospho-Tyr1092) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC
种属反应性Human;Rat
特异性The antibody detects endogenous level of EGFR only when phosphorylated at tyrosine 1092.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of tyrosine1092 (P-E-Y(p)-I-N) derived from Human EGFR.
基因/蛋白名称EGFR
标记Unconjugated
别名ERBB1; Receptor protein-tyrosine kinase ErbB-1; kinase EGFR
数据库入口号Swiss-Prot: P00533
NCBI Protein: NP_005219.2
Uniprot
P00533
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 175kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from A431 cells untreated or treated with EGF using EGFR(Phospho-Tyr1092) Antibody #11081.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR(Phospho-Tyr1092) Antibody #11081(left) or the same antibody preincubated with blocking peptide(right).
Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.
Buerger C, et al. (2003) J Biol Chem; 278(39): 37610-21.
Wang XQ, (2003) J Biol Chem; 278(49): 48770-8.
Saito T, et al. (2004) Endocrinology; 145(9): 4232-43.
Pao W, et al. (2004) Proc Natl Acad Sci U S A; 101(36): 13306-11.
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et al,Stiff substrates increase YAP-signaling-mediated matrix metalloproteinase-7 expression. In Oncogenesis on 2015 Sep 7 by A Nukuda 1 , C Sasaki et al..PMID:26344692
, (2015),
PMID:
26344692
et al,The EGF/EGFR axis and its downstream signaling pathways regulate the motility and proliferation of cultured oral keratinocytesInFEBS Open BioOn2023 AugbyRyota Kobayashi, Emi Hoshikawa et al..PMID:37243482
, (2023),
PMID:
37243482
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