产品详情
产品名称Tau(Phospho-Ser235) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of Tau only when phosphorylated at serine 235.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of serine 235 (P-K-S(p)-P-S) derived from Human Tau.
基因/蛋白名称Tau
标记Unconjugated
别名MAPT; MTAPT; MTBT1; Neurofibrillary tangle protein; PHF-tau
数据库入口号Swiss-Prot: P10636
NCBI Protein: NP _001116538.1
Uniprot
P10636
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 48 62 78 kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from mouse brain tissue using Tau(Phospho-Ser235) Antibody #11106 and the same antibody preincubated with blocking peptide.
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Alonso Adel C, et al. (2004) J Biol Chem. 279(33): 34873-34881.
Liu F, et al. (2002) FEBS Lett. 530(1-3): 209-214.
Sengupta A, et al. (1998) Arch Biochem Biophys. 357(2): 299-309.
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et al,The formation of tau pathological pathological phospho-epitopes in the axon is prevented by the dephosphorylation of selective sites in prinmary hippocampal neurons over-expressing human tau.
, (2010),
PMID:
20550628
et al,The formation of tau pathological phospho鈥恊pitopes in the axon is prevented by the dephosphorylation of selective sites in primary hippocampal neurons over鈥恊xpressing
human tau. In J Neurochem on 2010 Sep 1 by Johanne Bertrand, Patrick S茅n茅chal, et al..PMID: 20550628
, (2010),
PMID:
20550628
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