产品详情
产品名称Rb(Phospho-Ser780) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of Rb only when phosphorylated at serine 780.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of serine 780 (T-L-S(p)-P-I) derived from Human Rb.
基因/蛋白名称Rb
标记Unconjugated
别名P105-RB; PP105; PP110; RB-1; RB1
数据库入口号Swiss-Prot: P06400
NCBI Protein: NP_000312.2
Uniprot
P06400
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 110kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 3T3 cells untreated or treated with UV using Rb(Phospho-Ser780) Antibody #11132.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb(Phospho-Ser780) Antibody #11132(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using Rb(Phospho-Ser780) Antibody #11132.
Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Roesch A, et al.(2005) Mod Pathol. 18(4): 565-572.
Huang H, et al.(2004)Oncogene. 23(12): 2161-2176.
Kitagawa M, et al.(1996)EMBO J. 15(24): 7060-7069.
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