产品详情
产品名称CDC2(Phospho-Thr161) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of CDC2 only when phosphorylated at threonine 161.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of threonine161 (T-Y-T(p)-H-E) derived from Human CDC2.
基因/蛋白名称CDC2
标记Unconjugated
别名CDC28; CDC2A; CDK1; Cyclin-dependent kinase 1;
数据库入口号Swiss-Prot: P06493
NCBI Protein: NP_001163877.1
Uniprot
P06493
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 34kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with UV(lane 2) using CDC2(Phospho-Thr161) Antibody #11134.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDC2(Phospho-Thr161) Antibody #11134(left) or the same antibody preincubated with blocking peptide(right).
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
Ukomadu C, et al.(2003) J Biol Chem; 278(7): 4840-6.
Morris MC, et al.(2002)J Biol Chem; 277(26): 23847-53.
Brown NR, et al.(1999)J Biol Chem; 274(13): 8746-56.
Liu Y, et al.(2004)J Biol Chem; 279(6): 4507-14.
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et al,Adaptive Gene Regulation of Pyruvate Dehydrogenase Kinase Isoenzyme 4 in Hepatotoxic Chemical-Induced Liver Injury and Its Stimulatory Potential for DNA Repair and Cell Proliferation.In J Recept Signal Transduct Res on 2011 Feb by Minori Dateki, Megumi Kunitomo,et al..PMID:21182459
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et al,Sinularin induces oxidative stress鈥恗ediated G2/M arrest and apoptosis in oral cancer cells.In Environ Toxicol on 2017 Sep by Yung-Ting Chang , Chang-Yi Wu,et al..PMID:28548367
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et al,Enhanced Proliferation of Bone Marrow Mesenchymal Stem Cells by Co-Culture With TM4 Mouse Sertoli Cells: Involvement of the EGF/PI3K/AKT Pathway .In Mol Cell Biochem on 2014 Aug byHuan Tian , Meijin Guo et al..PMID:24748323
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