产品详情
产品名称JAK1(Phospho-Tyr1034) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of JAK1 only when phosphorylated at tyrosine 1034.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of tyrosine 1034 (K-E-Y(p)-Y-T) derived from Human JAK1.
基因/蛋白名称JAK1
标记Unconjugated
别名Janus kinase 1
数据库入口号Swiss-Prot: P23458
NCBI Protein: NP_002218.2
Uniprot
P23458
浓度1.0mg/ml
配方Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
WB 1:200-1:1000; IHC 1:100-1:300; IF 1:50-200
Western Blot analysis of various cells using Phospho-JAK1 (Y1034) Polyclonal Antibody diluted at 1:500
Western blot analysis of lysates from A549 cells , using JAK1 (Phospho-Tyr1034) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,JAK1 (phospho Tyr1034) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of mouse-liver tissue. 1,JAK1 (phospho Tyr1034) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Tyrosine kinase of the non-receptor type, involved in the IFN-a/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
Zheng H, et al.(2005)Mol Cell Proteomics. 4(6):721-730.
Wang R, et al.(2003) Arch Biochem Biophys. 410(1): 7-15.
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