产品详情
产品名称FKHRL1(Phospho-Ser253) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of FKHRL1 only when phosphorylated at serine 253.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of serine 253(A-V-S(p)-M-D) derived from Human FKHRL1.
基因/蛋白名称FKHRL1
标记Unconjugated
别名FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
数据库入口号Swiss-Prot: O43524
NCBI Gene ID: 2309
NCBI mRNA: NM_001455.3
NCBI Protein: NP_001446.1
Uniprot
O43524
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C
应用详情
Predicted MW: 97kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from NIH/3T3 cells using FKHRL1 (Ab-253) antibody (#21171, Lane 1 and 2) and FKHRL1 (phospho-Ser253) antibody (#11157, Lane 3 and 4).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHRL1 (phospho-Ser253) antibody (#11157).
Immunofluorescence staining of methanol-fixed HeLa cells using FKHRL1 (phospho-Ser253) antibody (#11157, Red).
Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.
Lehtinen M.K., Yuan Z., Boag P.R., Yang Y., Villen J., Becker E.B.E., DiBacco S., de la Iglesia N., Gygi S.P., Blackwell T.K., Bonni A.Cell 125:987-1001(2006) Morishita D., Katayama R., Sekimizu K., Tsuruo T., Fujita N.Cancer Res. 68:5076-5085(2008)
Kress T.R., Cannell I.G., Brenkman A.B., Samans B., Gaestel M., Roepman P., Burgering B.M., Bushell M., Rosenwald A., Eilers M.Mol. Cell 41:445-457(2011)
如果您使用该产品11157发表了文章，请通知我们，让我们可以引用您的文献。
et al,Activation of Akt/FoxO signaling pathway contributes to induction of neuroprotection against transient global cerebral ischemia by hypoxic pre-conditioning in adult rats.In J Neurochem on 2010 Aug by Zhan L, Wang T, et al..PMID:20492357
, (2010),
PMID:
20492357
et al,Activation of Akt/FoxO and inactivation of MEK/ERK pathways contribute to induction of neuroprotection against transient global cerebral ischemia by delayed hypoxic postconditioning in adult rats.In Neuropharmacology on 2012 Oct by Zhan L, Li D,et al..PMID:22749925
, (2012),
PMID:
22749925
et al,Involvement of PI3K/Akt/FoxO3a and PKA/CREB signaling pathways in the protective effect of fluoxetine against corticosterone-induced cytotoxicity in PC12 cells.In J Mol Neurosci
on 2016 Aug by Bingqing Zeng , Yiwen Li et al ..PMID:27412469
, (2016),
PMID:
27412469
有货
