产品详情
产品名称VASP(Phospho-Ser239) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of VASP only when phosphorylated at serine 239.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of serine 238 (K-V-S(p)-K-Q) derived from Human VASP.
基因/蛋白名称VASP
标记Unconjugated
数据库入口号Swiss-Prot: P50552
NCBI Protein: NP_003361.1
Uniprot
P50552
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 50kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from serum-treated 293 and PMA-treated C6 cells using VASP(Phospho-Ser239) Antibody #11158.
Immunohistochemical analysis of paraffin-embedded human tonsil carcinoma tissue using VASP(Phospho-Ser239) Antibody #11158(left) or the same antibody preincubated with blocking peptide(right).
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
Wang HG, et al.
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et al,Influence of short-term refrigeration on collagen-dependent signalling mechanisms in stored platelets. In Cell Signal on 2024 Oct
by Julia Zeller-Hahn, Marius Bittl,et al..PMID:39048036
, (2024),
PMID:
39048036
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