产品详情
产品名称DNA PKcs(Phospho-Thr2609) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB
种属反应性Human
特异性The antibody detects endogenous level of DNA PKcs only when phosphorylated at threonine 2609.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of threonine 2609 (V-E-T(p)-Q-A) derived from Human DNA-PK.
基因/蛋白名称DNA PKcs
标记Unconjugated
别名DNPK1; PRKD; PRKDC; XRCC7; P460
数据库入口号Swiss-Prot: P78527
NCBI Protein: NP_001075109.1
Uniprot
P78527
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 450kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from K562 cells untreated(lane 1) or treated with hydroxyurea(lane 2) using DNA PKcs(Phospho-Thr2609) antibody #11167.
The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties
Chan DW, et al. (2002) Genes Dev. Sep 15; 16(18): 2333-2338
Ding Q, et al. (2003) Mol Cell Biol. Aug; 23(16): 5836-5848
Douglas P, et al. (2002) Biochem J. Nov 15; 368(Pt 1): 243-251
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et al,Pyruvate Kinase M2 Phosphorylates H2AX and Promotes Genomic Instability in Human Tumor Cells.In Oncotarget on 2017 Nov 17 by Li Xia, Kang Qin,et al..PMID: 29312595
, (2017),
PMID:
29312595
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