产品详情
产品名称PKCth(Phospho-Ser695) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC
种属反应性Human
特异性The antibody detects endogenous level of PKCth only when phosphorylated at serine 695.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of serine 695 (N-F-S(p)-F-M) derived from Human PKCth.
基因/蛋白名称PKCth
标记Unconjugated
别名KPCT; PKC-theta; PKCQ; PRKCQ; PRKCT
数据库入口号Swiss-Prot: Q04759
NCBI Protein: NP_006248.1
Uniprot
Q04759
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 80kd
Western blotting: 1:500
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293 cells untreated or treated with PMA using PKCth(Phospho-Ser695) Antibody #11173.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCth(Phospho-Ser695) Antibody #11173(left) or the same antibody preincubated with blocking peptide(right).
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Xu ZB, et al.(2004) J Biol Chem 279:50401-50409
Thebault S, et al. (2004) Mol Immunol 40: 931-942
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