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位置: 首页 > 磷酸化抗体 > ASK1(Phospho-Ser83) Antibody

ASK1(Phospho-Ser83) Antibody#11178

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产品详情

产品名称ASK1(Phospho-Ser83) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Human

特异性The antibody detects endogenous level of ASK1 only when phosphorylated at serine 83.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 83 (G-S-S(p)-V-G) derived from Human ASK1.

基因/蛋白名称ASK1

标记Unconjugated

别名ASK-1; M3K5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5

数据库入口号Swiss-Prot: Q99683
NCBI Protein: NP_005914.1

Uniprot Q99683

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 155kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from K562 cells using ASK1(Phospho-Ser83) Antibody #11178(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1(Phospho-Ser83) Antibody #11178(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of extracts from 3T3 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using ASK1 (Phospho-Ser83) Antibody #11178.

Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.

Mabuchi S, et al. (2004) Endocrinology. 145(1): 49-58.
Yuan ZQ, et al. (2003) J Biol Chem. 278(26): 23432-23440.
Kim AH, et al. (2001) Mol Cell Biol. 21(3): 893-901.

如果您使用该产品11178发表了文章,请通知我们,让我们可以引用您的文献。

et al,ASK1-JNK signaling cascade mediates Ad-ST13-induced apoptosis in colorectal HCT116 cells. , (2010),
PMID: 20512919
et al,ASK1鈥揓NK signaling cascade mediates Ad鈥怱T13鈥恑nduced apoptosis in colorectal HCT116 cells. In J Cell Biochem on 2010 Jun 1 by Min Yang, Mingcan Yu, et al..PMID:20512919 , (2010),
PMID: 20512919

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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