产品详情
产品名称p27Kip1(Phospho-Thr187) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC
种属反应性Human
特异性The antibody detects endogenous level of p27Kip1 only when phosphorylated at threonine 187.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of threonine 187 (E-Q-T(p)-P-K) derived from Human p27Kip1.
基因/蛋白名称p27Kip1
标记Unconjugated
别名CDKN1B; CDN1B; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; KIP1
数据库入口号Swiss-Prot: P46527
NCBI Protein: NP_004055.1
Uniprot
P46527
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 27kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela cells untreated or treated with EGF, IFN-a using p27Kip1(Phospho-Thr187) Antibody #11208.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p27Kip1(Phospho-Thr187) Antibody #11208(left) or the same antibody preincubated with blocking peptide(right).
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.
Eguchi H, et al. (2003) Cancer Res; 63(15): 4739-46
Le XF, et al. (2003) J Biol Chem;
Connor MK, et al. (2003)
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et al,Insights in dynamic kinome reprogramming as a consequence of MEK inhibition in MLL-rearranged AML.In Leukemia on 2014 Mar by K R Kampen, A Ter Elst et al..PMID:24240200
, (2014),
PMID:
24240200
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