产品详情
产品名称b-Catenin(Phospho-Ser33) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of b-Catenin only when phosphorylated at serine 33.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of serine 33 (L-D-S(p)-G-I) derived from Human b-Catenin.
基因/蛋白名称b-Catenin
标记Unconjugated
别名CTNNB1; CATNB; CTNB1; CTNNB;
数据库入口号Swiss-Prot: P35222
NCBI Protein: NP_001091679.1
Uniprot
P35222
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 92kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from MCF-7 cells untreated or treated with Calyculin A using b-Catenin(Phospho-Ser33) Antibody #11218.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using b-Catenin(Phospho-Ser33) Antibody #11218(left) or the same antibody preincubated with blocking peptide(right).
Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.
Novak A, et al. (1998) Proc Natl Acad Sci U S A; 95(8): 4374-4379
Marin O, et al. (2003) Proc Natl Acad Sci U S A; 100(18): 10193-10200
Okamura H, et al. (2004) Mol Cell Biol; 24(10): 4184-4195
Xing Y, et al. (2003) Genes Dev; 17(22): 2753-2764
Barth AI, et al. (1999) Proc Natl Acad Sci U S A; 96(9): 4947-4952
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et al,Effect of Small-molecule GSK3 Antagonist on Differentiation of Rat Dental Pulp Cells into Odontoblasts.In In Vivo on 2020 May-Jun by Masuda Y, Sakagami H, et al..PMID: 32354894
, (2020),
PMID:
32354894
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