产品详情
产品名称EGFR(Phospho-Tyr869) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of EGFR only when phosphorylated at tyrosine 869.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of tyrosine 869 (K-E-Y(p)-H-A) derived from Human EGFR.
基因/蛋白名称EGFR
标记Unconjugated
别名ERBB1; Receptor protein-tyrosine kinase ErbB-1; kinase EGFR
数据库入口号Swiss-Prot: P00533
NCBI Protein: NP_005219.2
Uniprot
P00533
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 175kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from MDA cells untreated or treated with EGF using EGFR(Phospho-Tyr869) Antibody #11229.
Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.
Inoue A, et al. (2005) PLoS Med; 2(1): e13
Sun H, et al. (2004) EMBO J; 23(1): 100-110
Kanner SB, et al. (1991) Mol Cell Biol; 11(2): 713-720
Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674
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et al,EGFR-Phosphorylated Platelet Isoform of Phosphofructokinase 1 Promotes PI3K Activation. IN Mol Cell.On 2018 Apr 19 by Lee JH, Liu R et al..PMID:29677490
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et al,Secreted and O-GlcNAcylated MIF binds to the human EGF receptor and inhibits its activation.In Nat Cell Biol on 2015 Oct by Yanhua Zheng, Xinjian Li et al..PMID:26280537
, (2015),
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et al,Mutual regulation between phosphofructokinase 1 platelet isoform and VEGF promotes glioblastoma tumor growth. In Cell Death Dis on 2022 Nov 26 by Je Sun Lim, YuJie Shi, et al..PMID:36435833
, (2022),
PMID:
36435833
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