产品详情
产品名称IRS-1(Phospho-Ser636) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total IRS-1 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of serine 636 (P-M-S(p)-P-K) derived from Human IRS-1.
基因/蛋白名称IRS-1
标记Unconjugated
别名IRS-1; IRS1;
数据库入口号Swiss-Prot: P35568
NCBI Protein: NP_005535.1
Uniprot
P35568
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 180kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 3T3 cells untreated or treated with Insulin using IRS-1(Phospho-Ser636) Antibody #11230.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IRS-1(Phospho-Ser636) Antibody #11230(left) or the same antibody preincubated with blocking peptide(right).
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit
Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645
Tzatsos A, et al. (2006) Mol Cell Biol; 26(1): 63-76
Kadowaki T, et al. (2000) J Clin Invest; 106(4): 459-465
Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645
Szanto I, et al. (2000) Proc Natl Acad Sci U S A; 97(5): 2355-2360
如果您使用该产品11230发表了文章，请通知我们，让我们可以引用您的文献。
et al,The role of mitochondrial oxidative stress in the metabolic alterations in diet-induced obesity in rats. In FASEB J on 2019 Nov by Mar n-Royo G, Rodr guez C, et al..PMID:31370681
, (2019),
PMID:
31370681
et al,Metabolic inflammation exacerbates dopaminergic neuronal degeneration in response to acute MPTP challenge in type 2 diabetes mice.In Exp Neurol.On 2014 Jan by Wang L, Zhai YQ et al..PMID:24220636
, (2014),
PMID:
24220636
有货
