产品详情
产品名称SP1(Phospho-Thr739) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous levels of SP1 only when phosphorylated at threonine 739.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of threonine 739 (T-A-T(p)-P-S) derived from human SP1
基因/蛋白名称SP1
标记Unconjugated
别名TSFP1; Sp1 transcription factor;
数据库入口号Swiss-Prot: P08047
NCBI Protein: NP_612482.2
Uniprot
P08047
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 90kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from 3T3 cells treated with starvation using SP1(Phospho-Thr739) Antibody #11241.
Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-a G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.
Thiesen HJ,et al.(1990)Nucleic Acids Res.18(11):3203-9.
Zutter MM,et al.(1997) Blood.90(2):678-89.
Lou Z,et al.(2005)Cancer Res.65(3):1007-17
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et al,Synergistic effects of c-Jun and SP1 in the promotion of TGF尾1-mediated diabetic nephropathy progression.In Exp Mol Pathol on 2016 Jun by Pan Gao, Yingze Wei et al..PMID:27112839
, (2016),
PMID:
27112839
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