产品详情
产品名称p70 S6 Kinase(Phospho-Thr421) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of p70 S6 Kinase only when phosphorylated at threonine 421.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of threonine 421 (P-R-T(p)-P-V) derived from Human p70 S6 Kinase.
基因/蛋白名称p70 S6 Kinase
标记Unconjugated
别名KS6B1; P70-S6K; RPS6KB1; S6K;
数据库入口号Swiss-Prot: P23443
NCBI Protein: NP_003152.1
Uniprot
P23443
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 70 85 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Jurkat cells untreated(lane 1) or treated with GM-CSF(lane 2) using p70 S6 Kinase(Phospho-Thr421) Antibody #11254.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase(Phospho-Thr421) Antibody #11254(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF cells using p70 S6 Kinase(Phospho-Thr421) Antibody #11254.
Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.
Xiao-Feng, et al. (2003) Le1 30 Volume 22: 484-497
An WL, et al. (2003) Am J Pathol. 163(2): 591-607.
Le XF, et al. (2003) Oncogene.22(4): 484-97
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et al,Dauricine inhibits insulin-like growth factor-I-induced hypoxia inducible factor 1alpha protein accumulation and vascular endothelial growth factor expression in human breast cancer cells.
, (2009),
PMID:
19349962
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