产品详情
产品名称MARCKS(Phospho-Ser162) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of MARCKS only when phosphorylated at serine 162.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of serine 162 (K-K-S(p)-F-K) derived from Human MARCKS.
基因/蛋白名称MARCKS
标记Unconjugated
别名MACS; MARCS; PKCSL; PRKCSL; Protein kinase C substrate
数据库入口号Swiss-Prot: P29966
NCBI Protein: NP_002347.5
Uniprot
P29966
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 80kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 cells untreated or treated with PMA using MARCKS(Phospho-Ser162) Antibody #11265.
Immunofluorescence staining of methanol-fixed Hela cells using MARCKS(Phospho-Ser162) Antibody #11265.
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
Pariser H, et al. Proc Natl Acad Sci U S A 2005 Aug 30; 102(35): 12407-12412
Nagumo H, et al. Biochem Biophys Res Commun 2001 Jan 26; 280(3): 605-609
Yamamoto H, et al. Arch Biochem Biophys 1998 Nov 15; 359(2): 151-159
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et al,Delta Opioid Receptor Activation with Delta Opioid Peptide [d-Ala2, d-Leu5] Enkephalin Contributes to Synaptic Improvement in Rat Hippocampus against Global Ischemia.
In Cell Transplant on Jan-Dec 2021 by Guangming Zhang, Zelin Lai, et al..PMID:34470528
, (2021),
PMID:
34470528
et al,Phosphoproteome of signaling by ErbB2 in ovarian cancer cells. In Biochim Biophys Acta Proteins Proteom on 2022 Apr 1 by C Sidhanth, S Bindhya,et al..PMID:35158093
, (2022),
PMID:
35158093
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