产品详情
产品名称CREB(Phospho-Ser129) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of CREB only when phosphorylated at serine 129.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of serine 129 (I-L-S(p)-R-R) derived from Human CREB.
基因/蛋白名称CREB
标记Unconjugated
别名CREB-1; CREB1;
数据库入口号Swiss-Prot: P16220
NCBI Protein: NP _004370.1
Uniprot
P16220
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with UV(lane 2) using CREB(Phospho-Ser129) Antibody #11273.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB(Phospho-Ser129) Antibody #11273(left) or the same antibody preincubated with blocking peptide(right).
This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
Chrystelle V. Garat, et al. (2006) Mol. Cell. Biol ; 26: 4934 - 4948.
Lilah Rothem,et al. (2004) Mol. Pharmacol ; 66: 1536 - 1543.
Darren R. Tyson, et,al. (2002) Endocrinology; 143: 674.
Kyung-Woo Park, et al. (2003) Arterioscler. Thromb. Vasc. Biol ; 23: 1364.
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et al,IcariIn reduces high glucose?Induced endothelial progenitor cell dysfunction via InhibitIng the p38/CREB pathway and activatIng the Akt/eNOS/NO pathway. In Exp Ther Med on 2019 Dec by Chen S, Wang Z, et al..PMID:31772646
, (2019),
PMID:
31772646
et al,Small Molecule Inhibitors Targeting Gα i 2 Protein Attenuate Migration of Cancer Cells. In Cancers (Basel) on 2020 Jun 19 by Silvia Caggia, Subhasish Tapadar, et al..PMID: 32575572
, (2020),
PMID:
32575572
et al,Up-regulation of astrocyte excitatory amino acid transporter 2 alleviates central sensitization in a rat model of chronic migraine. In J Neurochem on 2020 Nov by Xue Zhou, Jie Liang, et al..PMID:31872442
, (2020),
PMID:
31872442
et al,Saikosaponin a alleviates depressive-like behavior by regulating the BDNF-TrkB-CREB signaling pathway in a mouse model of chronic unpredictable mild stress
, (2024),
PMID:
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