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位置: 首页 > 磷酸化抗体 > PKR(Phospho-Thr446) Antibody

PKR(Phospho-Thr446) Antibody#11280

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产品详情

产品名称PKR(Phospho-Thr446) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Human

特异性The antibody detects endogenous level of PKR only when phosphorylated at threonine 446.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 446 (K-R-T(p)-R-S) derived from Human PKR.

基因/蛋白名称PKR

标记Unconjugated

别名ADRB2; E2AK2; EIF2AK2; EIF2aK; PRKR

数据库入口号Swiss-Prot: P19525
NCBI Protein: NP_001129123.1

Uniprot P19525

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 68kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from K562 cells using PKR(Phospho-Thr446) Antibody #11280(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).

Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.

Abujiang Pataer, et,al. (2002) Cancer Res; 62: 2239.
K. D. Ryman, et,al. (2005) J. Virol; 79: 1487 - 1499.
Susana Guerra, et,al. (2006) J. Biol. Chem; 281: 18734 - 18745.
 

如果您使用该产品11280发表了文章,请通知我们,让我们可以引用您的文献。

et al,Airborne Particulate Matter Selectively Activates Endoplasmic Reticulum Stress Response in the Lung and Liver Tissues. , (2010),
PMID: 20554909
et al,Airborne particulate matter selectively activates endoplasmic reticulum stress response in the lung and liver tissues. In Am J Physiol Cell Physiol on 2010 Oct by Suzette Laing, Guohui Wang, et al..PMID: 20554909 , (2010),
PMID: 20554909
et al,Fluoxetine Inhibits NLRP3 Inflammasome Activation: Implication in Depression.In Int J Neuropsychopharmacol.On 2016 Sep 21 by Du RH, Tan J et al..PMID:27207922 , (2016),
PMID: 27207922
et al,Inhibition of the Integrated stress response by Epstein-Barr virus oncoprotein LMP1 attenuates epithelial cell differentiation and lytic viral reactivation. , (2025),
PMID: 39951426

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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