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CREB(Phospho-Ser142) Antibody#11300

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产品详情

产品名称CREB(Phospho-Ser142) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Human;Mouse;Rat

特异性The antibody detects endogenous level of CREB only when phosphorylated at serine142.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 142(D-L-S(p)-S-D) derived from Human CREB.

基因/蛋白名称CREB

标记Unconjugated

别名CREB

数据库入口号Swiss-Prot: P16220; NCBI Gene ID: 1385; NCBI mRNA: NM_004379.3; NCBI Protein: NP_004370.1

Uniprot P16220

实际分子量43KD

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C

应用详情
 Predicted MW: 43kd
 Immunohistochemistry: 1:50~1:100 
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB (Phospho-Ser142) Antibody (#11300).
Western blot analysis of extracts from 293 cells, treated with calf intestinal phosphatase (CIP), using CREB(Phospho-Ser142) Antibody #11300.

 Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

如果您使用该产品11300发表了文章,请通知我们,让我们可以引用您的文献。

et al,Gene expression pattern in PC12 cells with reduced PMCA2 or PMCA3 isoform: selective up-regulation of calmodulin and neuromodulin , (2012),
PMID: 21912933
et al,Methamphetamine Activates Trace Amine Associated Receptor 1 to Regulate Astrocyte Excitatory Amino Acid Transporter-2 via Differential CREB Phosphorylation During HIV-Associated Neurocognitive Disorders. In Front Neurol on 2020 Nov 25 by Irma E Cisneros, Anuja Ghorpade, et al..PMID:33324330 , (2020),
PMID: 33324330

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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