产品详情
产品名称Smad3(Phospho-Ser425) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of Smad3 only when phosphorylated at serine 425.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of serine 425 (C-S-S-V-S(p)) derived from Human Smad3.
基因/蛋白名称Smad3
标记Unconjugated
别名JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3
数据库入口号Swiss-Prot: P84022
NCBI Protein: NP_001138574.1
Uniprot
P84022
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 52kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela cells untreated or treated with EGF using Smad3(Phospho-Ser425) Antibody #11325.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Smad3(Phospho-Ser425) Antibody #11325(left) or the same antibody preincubated with blocking peptide(right).
Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
Shi W, et al. J Cell Sci. 2007 Apr 1;120(Pt 7):1216-24
Seong HA, et al. J Biol Chem. 2007 Apr 20;282(16):12272-89
Wordinger RJ, et al. Invest Ophthalmol Vis Sci. 2007 Mar;48(3):1191-200
LeClair RJ, et al. Circ Res. 2007 Mar 30;100(6):826-33
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et al,TGF β1 mediated Smad signaling pathway and EMT in hepatic fibrosis induced by Nano NiO in vivo and in vitro.In Environ Toxicol on 2020 Apr by Zhang Q, Chang X ,et al..PMID: 31737983
, (2020),
PMID:
31737983
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