产品详情
产品名称cofilin1/cofilin2(phospho-Tyr88) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用IHC WB
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of cofilin1/cofilin2 only when phosphorylated at tyrosine 88.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of tyrosine 88 (A-T-Y(p)-E-T) derived from Human coflin1/cofilin2.
基因/蛋白名称cofilin1/cofilin2
标记Unconjugated
别名CFL1/CFL2
数据库入口号Swiss-Prot: P23528 Q9Y281
NCBI Protein: NP _005498.1 NP _068733.1
Uniprot
P23528
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 19kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Mouse heart tissue using cofilin1/cofilin2(phospho-Tyr88) Antibody #11507.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin1/cofilin2(Phospho-Tyr88) Antibody #11507(left) or the same antibody preincubated with blocking peptide(right).
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
Carlier, M. et al. (1999) J. Biol. Chem. 274, 33827-33830.
Arber, S. et al. (1998) Nature 393, 805-809.
Yang, N. et al. (1998) Nature 393, 809-812.
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et al,DGCR6L, A Novel PAK4 Interaction Protein, Regulates PAK4-mediated migration of Human Gastric Cancer Cell via LIMK1.
, (2008),
PMID:
19778628
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