产品详情
产品名称PLCg1(phospho-Tyr771) Antibody
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of PLC-gamma1 only when phosphorylated at tyrosine 771.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of tyrosine 771 (P-D-Y(p)-G-A) derived from Human PLC-g1.
基因/蛋白名称PLCg1
标记Unconjugated
别名Phosphoinositide phospholipase C; Phospholipase C-gamma-1;
数据库入口号Swiss-Prot: P19174
NCBI Protein: NP_002651.2
Uniprot
P19174
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 155kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 3T3 cells untreated or treated with serum using PLC-g1(phospho-Tyr771) Antibody #11523.
Immunofluorescence staining of methanol-fixed Hela cells using PLC-g1(phospho-Tyr771) Antibody #11523.
PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
Yue, C. et al. (1998) J. Biol. Chem. 273, 18023-18027.
Margolis, B. et al. (1989) Cell 57, 1101-1107.
Yue, C. et al. (2000) J. Biol. Chem. 275, 30220-30225.
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et al,Discovery of a Novel Src Homology-2 Domain Containing Protein Tyrosine Phosphatase-2 (SHP2) and Cyclin-Dependent Kinase 4 (CDK4) Dual Inhibitor for the Treatment of Triple-Negative Breast Cancer
, (2022),
PMID:
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