产品详情
产品名称Cyclin B1(phospho-Ser147) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC IF
种属反应性Human
特异性The antibody detects endogenous level of Cyclin B1 only when phosphorylated at serine 147.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of Serine 147 (A-F-S(p)-D-V) derived from Human Cyclin B1.
基因/蛋白名称Cyclin B1
标记Unconjugated
数据库入口号Swiss-Prot: P14635
NCBI Protein: NP_114172.1
Uniprot
P14635
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from MDA cells untreated or treated with Anisomycin using Cyclin B1(phospho-Ser147) Antibody #11540.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1(Phospho-Ser147) Antibody #11540(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using Cyclin B1(phospho-Ser147) Antibody #11540.
The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
Norbury, C. and Nurse, P. (1992) Annu. Rev. Biochem. 61, 441-470.
Atherton-Fessler, S. et al. (1993) Mol. Cell. Biol. 13, 1675-1685.
Galaktionov, K. et al. (1995) Genes Dev. 9, 1046-1058.
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