产品详情
产品名称Cyclin E1(phospho-Thr395) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB
种属反应性Human
特异性The antibody detects endogenous level of Cyclin E1 only when phosphorylated at threonine 395.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of threonine 395 (L-L-T(p)-P-P)derived from Human Cyclin E1
基因/蛋白名称Cyclin E1
标记Unconjugated
别名CCNE; CCNE1;
数据库入口号Swiss-Prot: P24864
NCBI Protein: NP_001229.1
Uniprot
P24864
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 48kd
Western blotting: 1:1000
Western blot analysis of extracts from 293 cells untreated or treated with hydroxyurea using Cyclin E1(phospho-Thr395) Antibody #11541.
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition.
Won K.A., Reed S.I.EMBO J. 15:4182-4193(1996)
Welcker M., Singer J., Loeb K.R., Grim J., Bloecher A., Mol. Cell 12:381-392(2003)
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